Androgenic haploid plant development via embryogenesis with simultaneous determination of bioactive metabolites in Cambod tea (Camellia assamica ssp. lasiocalyx)

This pioneering work reports successful androgenic plant development via embryogenesis from microspore calluses in anther cultures and estimation of bioactive metabolites vitro regenerants parent (control) Cambod tea, Camellia assamica ssp. lasiocalyx (Planch MS) cultivar TV19. Anthers bearing microspores at early-to-late uni-nucleate stage were selected to initiate androgenesis. A pre-treatment 5 °C for days the dark was most effective profusely growing white callusing within 10 weeks culture on MS medium (6% sucrose) supplemented with high cytokinin/auxin ratio maintained by 6-benzylaminopurine (BAP) 2,4-dichlorophenxoyacetic acid. Nodular structures callus surface differentiated into embryos. Further developement embryos occurred but, ten times reduced concentration growth regulators additives. Germination complete plantlets achieved when major salts half (½ augmented BAP, GA3 IBA along glutamine serine. Cytological examination root-tip cells revealed that regenerated haploids (2n = x 15), which further confirmed through flow cytometry. The hot-water extracts haploid calluses, field-grown donor utilized quantification (+)-catechin, (−)-epicatechin, (−)-epigallocatechin gallate, caffeine theophylline. Our findings metabolite profile is comparable mother plant, thereby presenting them as potential source genome duplication genetically stable homozygous pure breeding lines. first report out-breeding tree, tea. It’s a significant achievement towards generating lines, impossible using conventional methods. Haploids showed consistent production.